摘要:本实验利用黑曲霉08013菌株发酵生产内切型菊粉酶，对发酵生产的工艺条件进行优化。首先测定菌株产酶的初始酶活为2.10U/mL。然后分别对不同装瓶量、pH、转速、温度进行单因素实验，确定发酵条件的最佳单因素分别为装瓶量80mL/250mL，pH7.0，转速160r/min，温度32℃。通过正交实验，得到了最佳工艺条件为：装瓶量80mL/250mL，pH7.0，32℃，80r/min摇床培养4d，在此条件下所测得的产酶活力最高，为5.24U/mL，酶活比条件优化前提高了149.14%。

关键词  黑曲霉；内切型菊粉酶；生产工艺；优化

Abstract:This experiment makes use of strain 08013 of Aspergillus niger to ferment and produce endo-inulinase, and optimizes the technique of fermentation and production. First, determine the initial enzyme activity of enzyme produced by strain. The enzyme activity is 2.10U/mL. Then, do single factor experiments at different bottle quantity, pH, revolving speed, temperature, respectively. Define the best single factor of the fermentation conditions are bottle quantity 80mL/250mL, pH7.0, revolving speed 160r/min, temperature 32℃, respectively. Lastly, by orthogonal experiment, determine that the best technical conditions are bottle quantity 80mL/250mL, pH7.0, temperature 32℃, revolving speed 160r/min for 4 days in shaker. Under these conditions, the activity of inulinase gets highest, the enzyme activity is 5.24U/mL. It is 149.15% more than before optimization.

Keywords  Aspergillus niger  endo-inulinase  production technique  optimization