摘要:本实验在以苹果渣为主的半固体培养基中，利用醋酸杆菌进行醋酸发酵。对基础培养基配方中的各因素（葡萄糖、酵母膏、硫酸镁、磷酸二氢钾、无水乙醇以及苹果渣）进行了优化，用单因素和正交实验的方法得到培养基的最优配方为：葡萄糖4%；酵母膏5%；无水乙醇3%；苹果渣15%；磷酸二氢钾0.45%；硫酸镁0.03%。在最优条件下，观察醋酸杆菌在苹果渣中的产酸曲线，3d后的产酸量最高为49.1g/L。

关键词  醋酸杆菌；苹果渣；产酸量；培养条件优化；正交试验

Abstract:Acetobacter was used to fermentate in the apple pomace semisolid culture medium.The influence of factors in the basic culture medium (glucose, yeast extract, magnesium sulfate, potassium dihydrogen phosphate, anhydrous ethanol and apple pomace) were optimized. The optimal composition of medium were as follows: glucose 4%; yeast extract 5%; ethanol 3%; apple pomace 15%; KH2PO4 0.45%; MgSO4 0.03%. Under the optimal conditions, by observating acid yield curve of Acetobacter in apple pomace, the acid production was up to 49.1g/L after 3 days’ fermentation..

Keywords Acetobacter  Pomace  Acetic acid production  Optimization of culture conditions Orthogonal experiment