摘要：本文以香菇菌种及蛹虫草菌种为研究材料，采用液体混合发酵培养的方法在不同条件下对混合菌种进行培养，获得菌丝体，通过测定菌丝体的生物量对发酵条件进行优化，得出最佳最优培养条件。实验结果表明：香菇菌种及蛹虫菌种混合发酵的最佳条件为比例为蛹：香 1:2，温度为25℃，转速为130r/min，最优培养时间为13天。此时菌丝体生物量为23.852mg/ml，鲜味物质氨基酸较纯种有了明显的增长。

关键词  香菇；蛹虫草；混合发酵；鲜味物质；氨基酸

Abstract:The mushrooms strain and strain of Cordyceps militaris as research material, using the method of liquid mixed fermentation cultured under different conditions on the mixed strains were cultured, obtain mycelium, the conditions of fermentation were optimized by measuring the biomass of mycelium, obtains the best optimal culture conditions. The experimental results show that: the best conditions of mushrooms and worms spawn fermentation for the ratio of pupae: Sweet 1:1, temperature is 25 ℃, the speed of 130r/min, the optimal culture time 13 days. The mycelium biomass is 23.852mg/mL to change into each ml, umami amino acids than the purebred has increased obviously. 

Key words  Lentinus edodes  Cordyceps militaris  mixed fermentation   Umami substances  aminopheno